Age-Related Changes in the Amount of Lipids in Mouse Oocytes

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Using mice of the ICR strain, sudanophilic lipids, lipoids and neutral fats were histochemically detected in the oocytes immediately after ovulation, and the amounts were compared among 30-day-old, 60-to 90-day-old and 180- to 210-day-old ones. Sudan IV -positive lipids and Ciaccio-positive lipoids were demonstrated in all the oocytes as droplets of different sizes, showing no significant difference among different age groups. Concerning nile blue sulfate-positive neutral fats observed as droplets of varied sizes, none was detected in the oocytes of 30-day-old mice, but came to appear in 1.0 % of the oocytes from 60-to 90-day-old mice, and extended to 6.3 % of those from 180-to 210-day-old mice, even showing a significant difference between 180-to 210-day-old mice and 30-day-old or 60- to 90-day-old mice. These results show that neutral fats in mouse oocytes first come to exist when the animals become mature, and the percentages of neutral fat-containing oocytes tend to be higher with the advance of animals' ages; while the amounts of sudanophilic lipids and lipoids do not change with aging.
日本哺乳動物卵子学会の論文