Possible autocrine function of progesterone on hexokinase activity of mouse preimplantation embryo

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A possible function of progesterone (P4) produced by mouse embryo was investigated during preimplantation period. Four cell embryos were obtained from PMS-hCG treated ICR mice 56h after hCG administration and cultured in modified BWW. Hexokinase (HK) activity of the embryos increased 4 fold after 36hr culture period, i. e. from 2. 68±0.11 to 10.5±0.61 pmol NADPH/embryo/h (mean±SEM). Addition of P4 did not significantly increase HK activity. When P4 production of the embryo was inhibited by trilostane (TRL), a specific inhibitor of 3β-hydroxysteroid dehydrogenase, the elevation of HK activity was suppressed dose-dependently (TRL 200μM, 4.02±0.18), and recovered by P4 supplement (P4 0.5μg/ml, 7.54±0.46). The inhibition was dominant when TRL was added in the first 24h. Actinomycin-D (ACTD) and cycloheximide, which inhibited mRNA transcription and protein translation respectively, both inhibited the elevation of HK activity dose-dependently, and the inhibition by ACTD was also dominant when added in the first half. These results suggest that P4 play a role in the induction of HK enzyme protein at the transcriptional level perhaps through autocrine fashion.
日本哺乳動物卵子学会の論文