Scanning Electron Microscopic Study of Interspecific Chimaeric Rlastocvsts

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Interspecific chimaeric blastocysts (mouse-rat, mouse-hamster, rat-hamster) were produced in the present experiment.Feulgen staining of nuclei and scanning electron microscopic observations of the chimaeric embryos were undertaken to analyse the origin of trophoblast cells, cell distribution and the interaction between cells derived from different species.<BR>In Feulgen nuclear reaction, the cells derived from mouse, rat and hamster were distinguished by observation of its nuclear size, and the shade of stained nuclei in chimaeric embryos.The cells derived from mouse had large nuclei which were homogeneously stained. The cells derived from rat had various size of nuclei which were smaller than those derived from mouse and were unevenly stained.The cells derived from hamster had small nuclei which were homogeneously stained.<BR>Observation with SEM showed the difference among the cells derived from each species embryos in density of microvilli on the surface of trophoblast cells.Trophoblast cells of which surface was covered with few microvilli were derived from mouse embryo. Dense microvilli were found on the surface of the cells derived from rat embryo. The cells derived from hamster were covered with many microvilli which were thicker and shorter than those of mouse or rat.<BR>In chimaeric blastocysts, trophoblast cells derived from each embryos distributed in groups at random regardless of their polarity.
日本哺乳動物卵子学会の論文