Effect of amino acids and hormones on the ability of male pronucleus formation of in vitro matured oocytes

概要

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The ability of male pronucleous formation of hamster follicular oocytes matured in vitro was examined by using in vitro fertilization technique of zona-free oocytes.<BR>When hamster oocytes, matured in vitro in a modified Tyrode's solution (mTALP) without amino acids and hormones, were fertilized in vitro, any oocytes could not get the ability to support the development of male pronucleus.<BR>By incorporating 12 amino acids (alanine, arginine, cystidine, glutamine, histidine, isoleucine, leucine, methionine, phenylalanine, proline and valine) and hormones (LH, FSH, PRL and estradiol-17β) in the maturation medium, higher percent (65%) of 75 oocytes gained the ability to support the development of male pronucleus.
日本哺乳動物卵子学会の論文