Zonae pellucidae of salt-stored eggs: Their functions in sperm-zona interactions

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When mammalian eggs are placed in a highly concentrated solution of neutral salt, the egg cytoplasm shrinks to become a small spherule, whereas the non living zona pellucida remains morphologically unchanged. In this study we examined the function of zona pellucida of salt-stored eggs in about sperm-zona interactions.<BR>(1) Zona penetration by spermatozoa began about same time when salt-stored and living eggs were inseminated with 2hr preincubated (capacitated) spermatozoa in the same dish. Also the time course changes in the ratio of penetration (or fertilization) was almost same.<BR>(2) When 2hr preincubated (capacitated) hamster spermatozoa were mixed with salt-stored and living eggs, almost same number of spermatozoa attached to each type of zona. The number of spermatozoa tightly bound to the two types zona was also about the same.<BR>(3) Acrosome reaction inducing ability of the zona was well mainteined after storage of eggs in salt solution. The major biological and biochemical properties of the zona were retained in the salt solution, therefore salt-stored eggs can be used to study sperm-zona interactions as the substitute of living eggs.
日本哺乳動物卵子学会の論文