Changes in energy metabolism during maturation of mammalian oocytes, with special reference to the measurement of phosphofructokinase activity in single oocytes

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It is well known that in mammalian oocytes meiotic division is resumed by LH surge. Oocytes and preovulatory follicles from immature rats injected with PMS or PMS-hCG were used for studies of the changes in energy metabolism during oocyte maturation. The activity of phosphofructokinase, a rate-limiting enzyme in glycolysis, and ATP content in one oocyte were measured using an ultramicroassay called the enzymatic cycling method. They increased markedly in parallel with meiotic maturation after h CG injection. The activity of phosphofructokinase increased after PMS injection dissociated from meiosis, which may be mediated by production of estrogen within the follicles. Oocyte maturation should be considered meiotic and cytoplasmic separately. It is proposed that activated energy metabolism, that is, glucose metabolism, is so good an indicator for cytoplasmic maturation as germinal vesicle breakdown and polar body emission for meiotic maturation.
日本哺乳動物卵子学会の論文