Plasminogen Activator Activity of Early Stage Mouse Embryos

概要

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To study fibrinolytic activity of preimplantation embryos, microassay methods to quantify plasmiogen activator in a single embryo were developed. 1. Preimplantation mouse embryos of day 2 and day 3 (4 cell to unhatched blastocyst stage) were incubated in 2μl medium drops containing 0.1% fibrin and 2 cu/ml plasminogen for 24 hours. Day 2 and day 3 embryos gave complete liquefaction of the fibrin drops. The amount of plasmin produced by a single ovum was assayed with synthetic fluorogenic substrate, Boc-Glu-Lys-Lys-MCA, and was 0.215±0.041 mcu with day 3 morula and 0.335±0.039 mcu with day 3 blastocyst. Day 1 and day 4 to 5 embryos (uncleaved ova and hatched blasocysts, respectively), incubated in the same way, produced only partial liquefaction of the fibrin drops. 2. When applied directly on plasminogen-rich fibrin plates, day 3 ova (morulae) produced grossly recognizable fibrinolysis. No lysis was observed on application of day 3 ova on plasminogen-free fibrin plates. Five μl medium drops incubated with day 3 embryos were applied on both kinds of fibrin plates and gave similar results.
日本哺乳動物卵子学会の論文