Analysis of pH Response for Amphoteric Poly(O-methacryloyl-L-serine) and Interaction with Serum Protein by Fluorescence Spectroscopy.

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The amphoteric polymer having L-serine residues in the side chain as a pH responsible hydrogel component was prepared as follows: Lserine with protected amino and carboxyl roups, N-tert-butoxycarbonyl-O-methacryloyl-L-serine diphenylmethyl ester (Boc-SerMA-Ph2) and 2- (dansyloxy) ethyl methacrylate (DnsEMA) were copolymerized by a radical mechanism with 2, 2′-dimethyl-azobisisobutyrate in tetrahydrofuran at 60°C for 20 h. Deprotection of the resulting polymer was carried out in 80% trifluoroacetic acid aqueous solution to give amphoteric poly (SerMA) labeled with a dansyl group [poly (SerMA-co-DnsEMA) ]. The fluorescence spectral changes of a dansyl-labeled poly (SerMA) solution at pH ranging from 1.2 to 12.0 revealed that poly (SerMA) shows pH response. The interaction between a serum protein such as bovine serum albumin (Alb), human γ-globulin (Glo), or bovine serum fibrinogen (Fib), and poly (SerMA-co-DnsEMA) was also examined by means of fluorescence spectroscopy in a phosphate buffered solution at pH 5.7 for Alb and Fib, pH 6.2 for Glo. The interaction between Alb and poly (SerMA-co-DnsEMA) was observed, whereas no definite interaction was confirmed for Glo and Fib.
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