家蚕消化液の低分子物質の抗菌力

概要

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A main antibacterial activity in the digestive juice of silkworm larvae fed on mulberry leaves was investigated by using dialyzed digestive juice and ethyl acetate soluble fractions of digestive juice. In the above experiments, it was recognized that some low molecular substances in the digestive juice had a main antibacterial activity.As low molecular antibacterial substances from the digestive juice of silkworm larvae, caffeic acid (CA) and protocatechuic acid (PA) were isolated by KOIKE et al. (1978), and D(-)-2, 3-diaminopropionic acid, which WADA and TOYOTA (1965) already isolated from the digestive juice, was recognized as antibacterial substance by IIZUKA (1977). In these experiments the antibacterial activities of CA, PA, quinic acid and chlorogenic acid (ChA) were investigated in the synthetic medium adjusted to pH7.0 and pH10.0, respectively.As a result of these experiments, in the synthetic medium at pH10.0, 500ppm of CA shows evidently antibacterial activity, while ChA does not show the activity even in the medium containing 1000ppm. However, when 1000ppm of ChA was added to the medium containing 500ppm of CA, the antibacterial activity was extremely increased. This synergistic action must play an important part in the control of bacterial flora in the gut of silkworm larvae.
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