Separation and Determination of Hexachlorophene and 3, 4, 4′-Trichlorocarbanilide by liquid chromatography
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概要
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Separation and determination of Hexachlorophene and 3, 4, 4′-Trichlorocarbanilide by liquid chromatography, which were typical cosmetic germicides, was investigated.The optimum analytical conditions were; column 8mm in inside diameter and 100mm in length, adsorbent silica-gel (Wakogel C-200), eluents 2% acetic acid-ethanol (1:1) in n-hexane-isopropyl ether (1:1), flow rate 1.54ml/min and Ultraviolet Adsorption Detector with 300nm filter.It was possible to separate Hexachlorophene and 3, 4, 4′-Trichlorocarbanilide within 30min and determined results of known sample was satisfactory.
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