The effects of the antifebrile agent sulpyrine on the synthesis of DNA, RNA and protein in HeLa S3 cells at high temperature.

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The effects of sulpyrine (56. 2, 100, 178, 320, 562 μg/ml) on DNA, RNA, and protein synthesis in HeLa S3 cells at 36.7°C and at high temperature (38.8, 41.1°C) were investigated. The cells at various cell densities (2.0×104, 4.0×104 and 8.0×104 cells/ml) were inoculated into each well of 24-well plates, and after 24-h cultivation at 37°C, adherent cells were further cultured for 48 h at high temperature with or without sulpyrine. The synthesis of DNA, RNA and protein was inhibited toward high temperature in cases of cultivations without sulpyrine. The synthesis of DNA, RNA, and protein was inhibited in a concentration-dependent manner by the addition of sulpyrine at each temperature. DNA, RNA and protein-IC50 values (sulpyrine concentration inhibiting synthesis of DNA, RNA or protein by 50% relative to untreated cells) at each temperature showed a tendency to increase with the increase in cell density. DNA and protein-IC50 values at each cell density at 38.8°C were not significantly lower than those at 36.7°C. However, RNA-IC50 values at each cell density were significantly (p<0.005) decreased, depending on the temperature. RNA-IC50 values at a cell density of 4.0×104 per ml at 36.7°C and 38.8°C were decreased from 411.7μ15. 0 μg/ml to 221.0×23. 3 μg/ml, respectively. The RNA-1C50 value at 41.1°C was lower than that at 38.8°C. However, the RNA-IC50 value at 41.1°C was excluded from the discussion, because DNA, RNA, and protein synthesis was markedly depressed at that temperature. These results suggest that the inhibitory effect of sulpyrine on RNA synthesis in HeLa S3 cells is enhanced at high temperature.
日本生気象学会の論文

The effects of the antifebrile agent sulpyrine on the synthesis of DNA, RNA and protein in HeLa S3 cells at high temperature.

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日本生気象学会 | 論文

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