Characteristics of Secretory Immunoglobulin A Secretion from Rat Salivary Glands.

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Secretory immunoglobulin A (sIgA) is one of the antimicrobial factors in saliva. This study was thus designed to elucidate sIgA secretion in three kinds of rat saliva (whole, parotid and submandibular) in response to various stimulants. As a result, amounts of sIgA in total protein scored the highest in submandibular, parotid and whole saliva in response to clonidine, an α<SUB>2</SUB>-agonist among various types of stimulants. Clonidine-induced secretion was completely antagonized with pretreatment of yohimbine, an α<SUB>2</SUB>-receptor (Re) antagonist. Most sIgA in whole saliva was secreted from parotid and submandibular glands regardless of the type of stimulus. In silver-stained native polyacrylamide gel, low molecular bands, which were seen in whole saliva, were also present in parotid saliva. On the other hand, high molecular bands seen in whole saliva were also present in submandibular saliva. Three bands stained with antibody against α heavy chain were observed in clonidine-elicited saliva by Western blotting. Because molecular weights of these proteins were 335, 845 and 1, 530kDa, they seemed to be sIgA dimer, tetramer and octamer. They could be present in equal volumes in each saliva. Tetramer and octamer, but not dimer, of sIgA have a secretory component.
歯科基礎医学会の論文