COMPUTATIONAL ANALYSIS AND MODELING OF GENOMESCALE AVIDITY DISTRIBUTION OF TRANSCRIPTION FACTOR BINDING SITES IN CHIP-PET EXPERIMENTS
スポンサーリンク
概要
- 論文の詳細を見る
Advances in high-throughput technologies, such as ChIP-chip and ChIP-PET (Chromatin Immuno-Precipitation Paired-End diTag), and the availability of human and mouse genome sequences now allow us to identify transcription factor binding sites (TFBS) and analyze mechanisms of gene regulation on the level of the entire genome. Here, we have developed a computational approach which uses ChIP-PET data and statistical modeling to assess experimental noise and identify reliable TFBS for c-Myc, STAT1 and p53 transcription factors in the human genome. We propose a mixture probabilistic model and develop computational programs for Monte Carlo simulation of ChIP-PET data to define the background noise of the sequence clustering and to identify the probability function of specific DNA-protein binding in the eukaryotic genome. Our approach demonstrates high reproducibility of the method and not only distinguishes bona fide TFBSs from non-specific TFBSs with a high specificity, but also provides algorithmic and computational basis for further optimization of experimental parameters of the ChIP-PET method.
- 日本バイオインフォマティクス学会の論文
日本バイオインフォマティクス学会 | 論文
- Performance Improvement in Protein N-Myristoyl Classification by BONSAI with Insignificant Indexing Symbol
- A combined pathway to simulate CDK-dependent phosphorylation and ARF-dependent stabilization for p53 transcriptional activity
- A versatile petri net based architecture for modeling and simulation of complex biological processes
- XML documentation of biopathways and their simulations in Genomic Object Net
- Prediction of debacle points for robustness of biological pathways by using recurrent neural networks