FragQA: predicting local fragment quality of a sequence-structure alignment
スポンサーリンク
概要
- 論文の詳細を見る
Motivation.<BR>Although protein structure prediction has made great progress in recent years, a protein model derived from automated prediction methods is subject to various errors. As methods for structure prediction develop, a continuing problem is how to evaluate the quality of a protein model, especially to identify some well predicted regions of the model, so that the structure biology community can benefit from automated structure prediction. It is also important to identify badly-predicted regions in a model so that some refinement measurements can be applied to.<BR>Results.<BR>We present a novel technique FragQA to accurately predict local quality of a sequence-structure (i.e., sequence-template) alignment generated by comparative modeling (i.e., homology modeling and threading). Different from previous local quality assessment methods, FragQA directly predicts cRMSD between a continuously aligned fragment determined by an alignment and the corresponding fragment in the native structure. FragQA uses an SVM (Support Vector Machines) regression method to perform prediction using information extracted from a single given alignment. Experimental results demonstrate that FragQA performs well on predicting local quality. More specifically, FragQA has prediction accuracy better than a top performer ProQres [18]. Our results indicate that (1) local quality can be predicted well;(2) local sequence evolutionary information (i.e., sequence similarity) is the major factor in predicting local quality; and (3) structure information such as solvent accessibility and secondary structure helps improving prediction performance.
- 日本バイオインフォマティクス学会の論文
日本バイオインフォマティクス学会 | 論文
- Performance Improvement in Protein N-Myristoyl Classification by BONSAI with Insignificant Indexing Symbol
- A combined pathway to simulate CDK-dependent phosphorylation and ARF-dependent stabilization for p53 transcriptional activity
- A versatile petri net based architecture for modeling and simulation of complex biological processes
- XML documentation of biopathways and their simulations in Genomic Object Net
- Prediction of debacle points for robustness of biological pathways by using recurrent neural networks