A bioassay method for herbicidal compounds using barley mutants with dripping-wet leaves

概要

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The barley varieties investigated were two water-repellent (Haruna Nijo and OUJ-659) and two water-wettable varieties (OUL-055 and OUM-012). The amount of waxes on barley leaf surfaces varied among varieties and, in particular, among growth stages. The water-repellent varieties had more waxes than did the water-wettable ones, and those at the late growth stage (120d-old) retained more waxes than those at the early growth stage (7d-old). The amount of leaf waxes at 120d-old was 6.36 to 20.92mg/g dry weight, which was about two folds that of 7d-old (3.92-9.28mg/g dry weight). Although the chemical compositions of the waxes did not differ among barley varieties, they changed significantly among growth stages. The effectiveness of a surfactant (ST-5) in enhancing retention of dye solution on leaves differed among the barley varieties. The water-wettable and water-repellent varieties showed the highest retentions at 0.01% and 0.1% of the surfactant (v/v), respectively. The water-wettable varieties were more sensitive to foliar application of glufosinate irrespective to the surfactant addition.
日本雑草学会の論文