Identification of Toxoplasma gondii Tachyzoites and Bradyzoites by a Quantitative Competitive Polymerase Chain Reaction Method after the Acid Treatment.
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概要
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It was indicated that tachyzoites and bradyzoites of <I>Toxoplasma gondii</I> (<I>T. gondii</I>) defined by the susceptibility/resistance to acid treatment could be quantitatively analyzed by a quantitative competitive polymerase chain reaction method targeting SAG1 gene, specific for <I>T. gondii</I>. The tachyzoites were destroyed not only by pepsin-HCl but also by HCl or other acid solutions. The acid-induced destructions occurred under the pH conditions lower than pH 1.8. Tachyzoite DNA of <I>T. gondii</I> was easily destructed by acid treatment. These DNA destructions made it possible to differentiate between tachyzoites and bradyzoites by QC-PCR.
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