Effect of Storage with Chlorine Dioxide Solution on Cryptosporidian Oocyst.

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Effects of storage in chlorine dioxide solution (ClO2. Na2CO3, 1% and 2% w/v in water) and 2% sulfuric acid solution on oocysts of Cryptosporidium muris and chicken Cryptosporidium sp. were examined. Fresh oocysts were collected from the feces of infected mice or chickens within 1 hr of oocyst shedding. They were poured into 7-fold volume of chlorine dioxide solution, sulfuric acid solution or 2% potassium dichromate solution and stored at 4°C. After 1, 2, 3 weeks and 1 and 2 months, the stored oocysts were washed 6 times with water by centrifugation and 106 oocysts from each storage were inoculated to a mouse or a chicken. Inoculated animals were examined daily for oocyst discharge. When stored in 1% chlorine dioxide solution, C. muris and chicken Cryptosporidium oocysts remained viable and retained infectivity for at least 3 weeks. Viable oocysts began to decrease after 1 month in 1% chlorine dioxide solution storage, though similar number of oocysts were detected by the sugar flotation method. These results clarified that 1% chlorine dioxide solution was usefull as a storage medium to keep oocysts viable for at least 3 weeks and detectable for 2 months.
日本熱帯医学会の論文