Cultivation of Trypanosoma cruzi using human diploid cells.

概要

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Interaction between normal diploid cells which had been established from human lung tissue and <I>Trypanosoma cruzi</I> (T. c) stock newly isolated from an Ecuadorian patient with Chagas' disease was studied. Normal diploid cells were found to have susceptibility to invasion by T. c and capacity to support the parasite growth. Intracellular parasites multiplied with a doubling time of 16.9 hours, and after 4 days of infection, trypomastigotes were released into culture supernatant. Development of T. c was relatively synchronized in the infection with parasite : host cell ratio of 20 : 1, providing broad form trypomastigotes with purity of more than 95 per cent in culture supernatant on day 4. Amastigotes were observed during the later stage of cultivation, resulting from degeneration of host cells. Two morphologically distinct forms of trypomastigotes, broad and slender, were obtained. Broad form predominated at early stage of cultivation and then proportion of slender form gradually increased.
日本熱帯医学会の論文