INDIRECT FLUORESCENT ANTIBODY STAINING OF MALARIA PARASITES, PLASMODIUM FALCIPARUM, IN THE HUMAN BRAIN AND LIVER

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A 33 years old man died of cerebral malaria caused by Plasmodium falciparum infection. Indirect fluorescent antibody staining was conducted with the brain and liver tissues as antigen. Small block of the tissue was frozen rapidly in n-hexane at -70°C and sections of 7 microns were made by use of the cryostat. Sections were treated in acetone for 10 minutes at room temperature. A rabbit fluorescein-labelled anti-human globulin antibody was used at 40 fold dilution. A normal human serum reacted at 4 fold dilution. Sera from patients with past history of Plasmodium vivax infection reacted up to 64 fold dilution. The late trophozoites of Plasmodium falciparum in the tissues fluoresced in cytoplasm and not in nucleus. The brain tissue did not show intense specific fluorescence when phosphate buffered saline was used for serum and could be used for detecting and measuring antibody. However, the mrlaria parasites in the liver tissue fluoresced and indicated that globulin of the patient himself had reacted to the parasite beforehand in vivo.
日本熱帯医学会の論文