The Effect of Nasal Fluid on the Release of Major Allergens from Japanese Ceder (Cryptomeria japonica) Pollens.:(Cryptomeria japonica) POLLENS
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概要
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In Japanese cedar (Cryptomeria japonica: CJ) pollinosis, release of major allergens (Cry <I>j</I> 1 and Cry <I>j</I> 2) from CJ pollens in the nasal cavity and their incorporation into the nasal mucosa are essential for the onset of the disease. The effect of nasal fluid on the release of major allergens from the pollen grains was studied <I>in vitro</I>.<BR>CJ pollens were suspended in normal saline and nasal fluid solution (NFS), and the ratio of rupture of the pollen grain wall on a slide glass was determined under a light microscope. The concentration of protein eluted in the supernatant of the cedar pollen suspension was determined by modified <I>Lowry</I>'s protein assay. The major allergens of CJ in the supernatant were quantified by sandwich enzyme-linked immunosorbent assay (ELISA) using rabbit polyclonal antibody. The antigenicity of the pollen extract was examined by the proliferative response of mouse <I>Cry j</I> 2 specific helper T cell lines.<BR>Both the ratio of rupture of the pollen grains and the concentration of protein in the supernatant significantly increased in the presence of NFS. Elution of the major allergens also increased in the presence of NFS, and the amount of <I>Cry j</I> 1 was larger than <I>Cryj</I> 2. The study on various factors involved in elutionof major allergens suggested that elution is promoted by components of nasal fluid, such as proteins, mutins, and lysozyme, as well as such physicochemical factors as pH and temperature. In conclusion, this study indicates that CJ pollens are in the state that is easy to release the major allergens in the nasal cavity of patients with allergic rhinitis.
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