Lipid in the Brown Planthopper, Nilaparvata lugens STÅL(Hemiptera : Delphacidae) : I. Lipid Contents and Composition in Two Wing-Forms, Brachypterous and Macropterous

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Total lipid content and its constituent in the brown planthopper, Nilaparvata lugens, were analyzed by thin layer chromatography (TLC) and gas liquid chromatography (GLC). The planthoppers analyzed were newly emerged adults of both sexes of two wing-forms, brachypterous and macropterous. All of them were reared throughout their nymphal stage with young rice seedlings at 25°C, 14 hr illumination. Total lipid was extracted with 2 : 1 chloroform-methanol (v/v). Total lipid contents on a dry weight basis in a brachypterous male, macropterous male, brachypterous female and macropterous female were 23.5%, 30.4%, 21.4% and 30.9%, respecitively. The lipids were separated to neutral lipids (NL) and polar lipids (PL), and their constituents were determined by TLC. Hydrocarbon, triglyceride, free fatty acid, sterol, diglyceride and monoglyceride were found in NL, phosphatidylethanolamine, phosphatidylcholine, lysophosphatidyl-ethanolamine, sphingomyeline and lysophosphatidylcholine in PL. The fatty acid composition in NL, PL and triglyceride was analyzed by GLC. The main fatty acids were C16 : 0, C16 : 1, C18 : 0, C18 : 1 and C18 : 2 in all three classes of lipids. About 80% was occupied by C16 : 0 and C18 : 1 in NL and triglyceride, while more than 60% by only C18 : 2 in PL. Triglyceride was analyzed by GLC, and the carbon numbers of acyl moieties were determined to be 46, 48, 50, 52, 54 and 56.
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