Sequencing of the p143 gene encoding a putative DNA helicase of the Spodoptera exigua (Lepidoptera: Noctuidae) nucleopolyhedrovirus I1 isolate from Japan.
スポンサーリンク
概要
- 論文の詳細を見る
The p143 gene of the Spodoptera exigua multinucleocapsid nucleopolyhedrovirus (SeMNPV) Il isolate was cloned and sequenced. This process revealed a 3, 666-nucleotide open reading frame (ORF), which encodes a deduced 1, 222-amino acid polypeptide with a molecular weight of 143, 285 (P143). The SeMNPV 11 P143 possessed seven consensus motifs conserved in members of the superfamily of DNA or RNA helicases. Seven amino acid substitutions were identified, when compared with the P143 of the SeMNPV US isolate. Of the seven substitutions, six were localized within the amino acid residues from 484 to 547. The deduced amino acid sequences in this region were compared among five geographically distinct isolates after amplification by PCR. The results showed that these were identical with that of the I1 isolate. SeMNPV I1 P143 had approximately 40% identities and more than 66% similarities to other NPV DNA helicases. However, the carboxyl-terminus region including the consensus motifs was identical within the SeMNPV isolates and relatively conserved among four NPVs.
- 日本応用動物昆虫学会の論文
日本応用動物昆虫学会 | 論文
- I104 チャバネゴキブリにおける二つのアセチルコリンエステラーゼの組織局在
- B307 カイコ由来抗菌性タンパク質ディフェンシン遺伝子の発現解析
- B105 カブトムシディフェンシン由来改変ペプチドの癌細胞増殖抑制効果
- H303 ハスモンヨトウの抗菌ペプチドの探索と殺菌剤への応用(生理活性物質)
- F313 タイワンカブトムシデフェンシンの単離とクローニング(生理活性物質、形態学・組織学・遺伝学)