Replication of Bacillus thuringiensis in larvae of the Mediterranean flour moth, Ephestia kuehniella (Lepidoptera: Pyralidae): Growth, sporulation and insecticidal activity of parasporal crystals.

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Spores and parasporal crystals of a Bacillus thuringiensis serovar aizawai strain were fed to fifth instar larvae of the Mediterranean flour moth, Ephestia kuehniella, under laboratory conditions. Viable numbers of B. thuringiensis cells in E. kuehniella larvae were estimated by dilution-plating on peptone-polymyxin agar medium, and combined spore and crystal insecticidal activity was bioassayed with larvae of Bombyx mori. B. thuringiensis rapidly grew and sporulated in the larval cadavers of E, kuehniella regardless of inoculation dose. Spores that were produced in situ in E. kuehniella cadavers persisted for at least 90 d. Insecticidal activity of the homogenates of E. kuehniella cadavers fluctuated along with the viable cell numbers and persisted for at least 90 d. To examine horizontal transmission of B. thuringiensis in E. kuehniella larval populations, B. thuringiensis-infected and healthy larvae were released into containers in various proportions, but no reliable transmission was detected under our experimental conditions.
日本応用動物昆虫学会の論文