Suppression of Bacterial Wilt Disease of Tomato by Root-dipping with Pseudomonas fluorescens PfG32. The Role of Antibiotic Substances and Siderophore Production.:The Role of Antibiotic Substances and Siderophore Production

概要

論文の詳細を見る
Pseudomonas fluorescens strain PfG32 isolated from the rhizosphere of the onion actively suppressed the occurrence of bacterial wilt disease of tomato (BWT) in vermiculite amended natural soil and produced antibiotic substance(s) and siderophore. To investigate the role of the substances in the biocontrol, several mutants defective in antibiotic substances or Siderophore production were isolated from spontaneous Rifr mutant, PfG32R, by Tn5 insertion. Based on pot experiment, the suppression of BWT by Ant-Sid+ (antibiotics non-producing but siderophore producing) mutants was lower compared to its parental strain and to Ant+Sid- (siderophore non producing but antibiotics producing) mutants. However, Ant-Sid+ mutants still express a significant effect. The suppression of BWT by PfG32R was correlated to the suppression of the population of the pathogen observed on the surface of root and to the delay of appearance of detectable population of the pathogen in root tissues. We consider that the ability of PfG32 to produce antibiotics and siderophore is the important factor on determining its biological control and other factor may act as secondary factor.
日本植物病理学会の論文