Localization of tobacco mosaic virus in tobacco callus tissue.

概要

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Tobacco callus was raised from a stem of tobacco mosaic virus (TMV)-infected tobacco plant (cv. Bright Yellow). By means of successive transfers of green colored compact peripheral tissue, intermediate tissue between green and translucent tissue, and translucent soft inner tissue of the callus respectively on Murashige and Skoog's medium, the following 3 types were developed. Callus A; dark green colored callus composed of compactly arranged small cells. Callus B; green colored callus composed of compact peripheral tissue and translucent inner tissue. Callus C; translucent and soft callus composed of loosely arranged large cells.TMV concentration in tobacco callus declined by repeated transfers of translucent soft tissue, while it was maintained at a high level when green colored compact tissue was successively transferred. The distribution of TMV in relation to cell arrangement in the tobacco callus was investigated by means of fluorescent antibody technique. TMV antigen in tobacco callus was unevenly distributed, that is, it was frequently localized in compactly arranged small cells around tracheid-like cells, whereas it was rarely observed in soft tissue where large cells were loosely arranged. The decline of TMV concentration in the translucent soft callus was thought to be due to the rapid propagation of healthy cells escaped the virus infection.
日本植物病理学会の論文