Studies on Resistance of Wheat Varieties to Scab (Gibberella zeae (Schw.) Petch.) : 1. The method of inoculation to leaf blade.

概要

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The resistance of wheat varieties to scab can be estimated only after heading, because of the rare occurrence of this organism on leaf blade. The purpose of this study was to develope the practical method of inoculating scab to leaf blade for the use of breeding program. Though various difficulties were caused mainly by the requirements for both moisture and nutrient of this organism, the inoculation to leaf blade was achieved by the following ways: 1. A few little holes were pricked with a pin in a given part of leaf blade and inoculated with spores of scab. 2. A vinyl ring (4 mm in diameter, 3 mm in length), containing potato agar culture medium, was placed on the inoculated part of leaf blade, and adhered with cellotape. Furthermore, they were wrapped with wrap-film (4 cm × 4 cm) to avoid drying out. 3. The inoculated leaf was held with a rod, and thus treated plant was grown in the glasscovered thermostat at 18°C. After 3∼4 days, pale brown with yellow margins appeared on the inoculated leaf as a symptom of scab infection, and the area of the diseased part expanded gradually not only to upper but also to lower parts of the leaf blade. The relation between the degrees of the infection and the resistance remains to be determined.
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