Autoradiographic Studies on RNA and Protein Synthesis of Human Skin in vivo in Normal and Pathologic Conditions by 3H-uridine and 3H-leucine
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The RNA and protein synthesis of normal and psoriatic skins together with RNA and protein synthesis of eccrine, and apocrine sweat glands as well as sebaceous glands have been studied by 3H-compounds autoradiography. Tritiated uridine and tritiated leucine are administered by 'local labeling method'. The results obtained were as follows.1. The synthesis of RNA and protein was detected in all epidermal cells. The silver grains, indicating RNA and protein synthesis, were seen most dense in prickle cells, moderately dense in basal cells and poor in granular cells.2. Within one hour after 3H-uridine injection the 3H-labels which were incorporated in RNA of the epidermal cells were located on the nuclei, particularly on the nucleoli. Two or three hours after 3H-uridine injection the labels were found migrating to the cytoplasm.3. Between normal and psoriatic epidermis no differences of the intensities of RNA and protein synthesis were recognized in the cellular level. It is probable that the marked difference of the epidermis thickness was responsible for the difference of RNA and protein synthesis so far reported in normal and psoriatic conditions.4. In eccrine and apocrine sweat glands the RNA synthesis was found in all glandular epithelial cells. The labels were located on the nucleus of the cells within one hour after the injection.5. After 3H-leucine injection, the eccrine and the apocrine sweat glands showed diffuse labeling in all the glandular epithelial cells. In the apocrine gland, 30 minutes after the injection of 3H-leucine the labels were diffusely found within all the glandular cells. And 1.5 hours after the injection the labels became recognizable in the glandular lumen.6. One hour after injection of 3H-uridine the labels indicating RNA synthesis of sebaceous gland were located on the nuclei of all the inner maturative cells and all the generative cells that form external layer. Thirty minutes after 3H-leucine injection the labels were diffusely seen in the whole cell bodies of the sebaceous gland.7. Between normal and psoriatic skins no differences of RNA and protein synthesis in the eccrine and the sebaceous gland were recognizable.
- 国際組織細胞学会の論文
国際組織細胞学会 | 論文
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