In Vitro Propagation of Lagerstroemia spp. from Nodal Explants and Gaseous Composition in the Culture Headspace.
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概要
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Shoot multiplication and plantlets regeneration were achieved from single-nodal explants (with two unfolded leaves) of mature trees of <I>Lagerstroemia speciosa</I> Pers. and <I>Lagerstroemia thorellii</I> Gagnep. through in vitro culture. Nodal explants directly produced multiple shoots when cultured on Murashige and Skoog (Physiol. Plant. (1962) 15: 473-497) medium with 6-benzyl amino purine (BAP ; 1.5 mg L<SUP>-1</SUP>) and indole-3-acetic acid (IAA; 0, 1 mg L<SUP>-1</SUP>) . Cuttings, each having a shoot apex and a node obtained from regenerated shoots were cultured on fresh medium for further multiplication, and an average of 17-20 shoots per cutting were obtained. The differentiated shoots were rooted on half strength MS medium with 0.5 mg L<SUP>-1</SUP> indole-3-butyric acid (IBA) . The effects of different capping systems on growth during the in vitro rooting stage and percent survival of plants ex vitro, the concentrations of ethylene and CO<SUB>2</SUB> and the relative humidity in the culture headspace of <I>L. speciosa</I> were also studied. In sealed vessels high concentration of ethylene was observed, which was not the case under forced ventilation. In the photoperiod, the CO<SUB>2</SUB> concentration in the sealed vessels decreased to ca. 50 μL L<SUP>-1</SUP>; while the concentration was higher in the capping systems with natural ventilation and was highest in the vessels under forced ventilation compared to that of the sealed system. The relative humidity was nearly 100% in the sealed vessels and was 89% in the vessels under forced ventilation. The growth (leaf and root fresh and dry mass) and the percent of the survived plants in the ex vitro conditions were highest in the forced ventilation treatment.
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