The relative efficacy of toxic core fragments of three Bacillus thuringiensis insect control proteins against lepidopteran insects damaging turf and the efficacy of the Cry1Ac toxin against these insects plus an insect damaging rice
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The relative efficacy of toxic core fragments of three lepidopteran-specific<I>Bacillus thuringiensis</I>insect control proteins, Cry1Aa, Cry1Ab and Cry1Ac, were determined against two most damaging turf insects, <I>Pediasia teterrellus</I>and<I>Spodoptera, depravata</I>. The toxic core fragments of the three proteins were obtained through expression in<I>Escherichia coli</I>. The most toxic among the three was the fragment of Cry1Ac against both lepidopteran insects. The efficacy of the Cry1Ac toxin was also determined against these insects plus<I>Parnara guttata</I>. The level of this protein required for 50% motality (LC<SUB>50</SUB>) against<I>P. teterrellus, S. depravata</I>and<I>P.guttata</I>were 0.65ppm, 2.5ppm and 0.044 ppm, respectively.
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