Acute Toxicity of Ozone-Exposed Seawater and Chlorinated Seawater for Japanese Flounder, Paralichthys olivaceus, Eggs, Larvae and Juveniles.

概要

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The toxic effects of exposure to ozone-exposed seawater and chlorinated seawater were investigated in eggs and larvae aged 3-15 days and juveniles aged 44 days in the setting stage of Japanese flounder, Paralichthys olivaceus. For eggs, the 50% no-hatching concentration of OPO and TRC after 1 min exposure were both about 2.2 mg O3/l. The 24-h LC50 values of OPO and TRC in larvae aged 3-15 days were both 0.02-0.05 mg O3/l. The 24-h LC50 values of OPO and TRC for juveniles aged 44 days in the setting stage were both 0.14-0.15 mg O3/l. Light microscopy of branchial tissues showed detachment of the epithelium of the secondary lamellae after exposure to OPO or TRC. Scanning electron microscopy (SEM) of branchial tissues showed cell swelling and cell destruction of the secondary lamellae after exposure to OPO or TRC. The toxicity of OPO and TRC and damage to branchial tissue caused by OPO and TRC were exactly alike.
日本水産増殖学会の論文