Changes in the Concentrations of Ammonia and Particulate Organic Matter and Rotifer Biomass in High Density Semi-Continuous Culture.
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概要
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For the reliable process management of high density culture of marine rotifer <I>Brachionus rotundiformis</I>, it is very important to know the quantitative behavior of rotifer biomass, the concentrations of particulate organic matters including nutritional organic matters for rotifer, and total ammonia (NH<SUB>4</SUB>-N) . In this study, changes in dry weight, PV (packed volume) and population of rotifer, PV of particulate organic matters and concentrations of NH<SUB>4</SUB>-N in high density semicontinuous culture were investigated. High density culture was performed by supplying oxygen from a generator, controlling the pH at 6.7 and feeding condensed <I>Chlorella regularis</I> at a feed rate of 4.2 kg/day with a peristaltic pump. Filtration unit was also used to remove the excess amount of particulate organic matters (mainly bacterial flocs and films) from culture medium. Total amount of accumulated <I>Chlorella</I> (<I>Rn</I>, g) in the culture tank was expressed with the feed rate of <I>Chlorella</I> (<I>S</I>, g/day), culture day (<I>n</I>) and harvest rate (<I>T</I>) as follows.<BR><I>Rn</I>= {1- (1-<I>T</I>) <SUP>n</SUP>} / {1- (1-<I>T</I>) } ⋅<I>S</I><BR>The amount of NH<SUB>4</SUB>-N produced (<I>Yn</I>, g) and the concentration of NH<SUB>4</SUB>-N in culture medium (<I>An</I>, ppm) were estimated by following exprimental formulas. where <I>a</I> is the initial ammonia concentration (ppm) and <I>V</I> is working volume (k<I>l</I>) .<BR><I>Yn</I>= 0.0326 <I>Rn</I> (<I>r</I>=0.95, <I>p</I><0.01)<BR><I>An</I>=<I>a</I>⋅ (1-<I>T</I>) <SUP>n</SUP>+<I>Yn/V</I><BR>The result of partial correlation analysis for the PVs of rotifer, wastes layer, and microbial layer and culture day indicated that the wastes layer was utilized by rotifer as feed but microbial layer was not utilized. As the result of multiple regression analysis, it was proved that the measurement of PV was more reliable among the methods for quantitative determination of rotifer biomass. The measurement of PV can be easily performed by the centrifugation of culture medium, therefore PV monitoring will be very useful for on-line management of high density culture of rotifer. In the semicontinuous high density culture of 20 days, the feed conversion rate of <I>Chlorella</I> to rotifer was found to be 23.5% (w/w) . Dry weight and PV per rotifer individual were estimated to be 1.6 × 10<SUP>-7</SUP> g and 9.2 × 10<SUP>-7</SUP> m<I>l</I>, respectively, and those per rotifer egg were 0.9 × 10<SUP>-7</SUP> g and 4.9 × 10<SUP>-7</SUP> m<I>l</I>, respectively.
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