Role of whole blood luminol-dependent chemiluminescence on monitoring acute bacterial infections.

概要

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We have tested whether or not luminol-dependent chemiluminescence (CL) of whole blood can be used for monitoring an acute bacterial infection or acute episode of bacterial infection of patients with chronic infectious diseases. This assay has several serious problems to get a reliable and reproducible measurement, for example, diurnal and daily variation, changeable measurement by time after bleeding and contamination of red blood cells and plasma constituents. These shortcomings could be overcome by using 10μ<I>l</I> of whole blood instead of 100μ<I>l</I> of it and by measuring it within 30 minutes after bleeding at a certain fixed time for a patient. Acute bacterial infections, such as acute pneumonia or acute pleuritis induced a great increased CL response which turned to the normal level immediately after its recovery. The similar kinetics of CL response was observed at acute episode of patients with chronic infectious diseases. A very good correlation existed between CL response and clinical signs of acute bacterial infections, such as fever, increased erythrocyte sedimentation rate, leukocytosis (r=0.73), increased CRP (r=0.88) . This assay can be used as one of the rapid and simple function tests of leukocytes for monitoring acute bacterial infections.
日本炎症・再生医学会の論文