Studies on the phlogogenic effects of lysosomal fraction isolated from PMN leukocytes:Comparison of the PMN leukocyte fraction from peripheral blood with that from rheumatoid synovial fluid

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The phlogogenic effects of lysosomal fraction isolated from PMN leukocytes of peripheral blood were compared with that of rheumatoid synovial fluid PMN leukocytes. Leukocyte lysosomes contain materials which increased vascular permeability and provoked inflammation (ex. rubor and induration) on the rabbit skin. We recognized that the rubor was larger by injected with the peripheral blood PMN lysosomal fractions than by the same fractions from synovial fluid PMN, while the induration was more intense by the lysosomal fractions from synovial fluid PMN than by that from the peripheral blood PMN. Fresh human serum treated with lysosomal fractions from peripheral blood, or synovial fluid PMN induced cytochalasin B-treated PMN to release β-glucuronidase, but the enzyme releasing activity was not significantly different by the source of lysosomal fraction. The supernatant of normal PMN reacted with NHS and PMN lysosomal fractions has a chemotactic activity, and the activity was 2 times higher by the synovial fluid PMN than by the peripheral blood PMN lysosomal fractions, explaining the more intense induration produced by the synovial fluid PMN lysosomal fractions.
日本炎症・再生医学会の論文