The enhancing effect of .BETA.-cyclodextrin on IL-1 production from peritoneal macrophages in mice .

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Cyclodextrin (Cd) is a cyclic oligosaccharide and can include various kinds of compounds in its cavity. Cd applications improve the bioactivity of the compounds. Recently, it has been clarified that some oligosaccharides are immunostimulating agents, therefore, we studied the effects of Cds on IL-1 production from macrophages in mice. The addition of Cd increased IL-1 production in the cultured supernatants from LPS-stimulated macrophages of BALB/c mice.<BR>Among three kinds of Cd, βCd was the most effective and γCd was less, but αCd did not show any effect at all. In C3H/HeJ mice, a similar enhancing effect using the 3 kinds of Cd was observed, however, it was much weaker. IL-1 production did not increase when the macrophages were precultured with βCd and later stimulated by LPS.<BR>Using ELISA, we found that IL-1 molecules in the cultured supernatants was increasing. βCd induced more strongly the IL-1 mRNA expression of LPS-stimulated macrophages. The lymphocyte activating factor activity of recombinant IL-1 was not stimulated in the presence of βCd.<BR>These results indicate that Cd enhances IL-1 production from LPS stimulated macrophages, although Cd itself does not induce the IL-1 syntheses, which is associated with the formation of the inclusion complex of Cd with LPS and the increase of LPS solubility in water.
日本炎症・再生医学会の論文