Expression of apoptosis-associated antigens in synovial tissues of rheumatoid arthritis.
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We examined whether apoptosis-associated antigens were expressed in synovial tissues from nine patients with rheumatoid arthritis (RA) and five patients with osteoarthritis (OA) using immunohistochemical staining and DNA nick end labeling.<BR>Paraffin sections of synovial tissues were stained with various antibodies, such as anti-PCNA monoclonal antibody, anti-Fas monoclonal antibody, anti-Le<SUP>y</SUP> monoclonal antibody and anti-<I>bcl-2</I> polyclonal antibody. DNA nick end labeling was also performed on each of the paraffin sections.<BR>In RA specimens, the PCNA positivity ratio and Fas positivity ratio of synovial lining cells were 16.85±4.28% and 1.97±0.99%, respectively. On the other hand, in OA the corresponding ratios were 4.25±0.66% and 1.58±1.24%, respectively. The degree of proliferation of synovial lining cells was significantly different between RA and OA, but Fas antigens on the synovial lining cells did not differ significantly. DNA nick end labeling and staining with anti-Fas monoclonal antibody were positive in synovial lining cells, macrophages and fibroblasts. Anti-Le<SUP>y</SUP> monoclonal antibody produced positive staining in inflammatory cells. On the other hand, anti<I>bcl</I>-2 polyclonal antibody gave a positive reaction in synovial lining cells, macrophages, fibroblasts, inflammatory cells and vascular endothelial cells.<BR>We conclude that apoptosis-associated molecules (for enhancement and inhibition) are expressed in synovial tissues from patients with RA.
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