Induction of IL-1.BETA. mRNA in human gingival fibroblasts by adhesive interaction to lymphoid cells.

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Chronic marginal periodontitis is characterized by dense infiltration of lymphocytes in periodontal connective tissue. In this study, we investigated whether adhesive interactions between human gingival fibroblasts (HGF) and lymphoid cells may cause activation of the HGF. <I>In vitro</I> cell lines of HGF were co-cultured with human lymphoid cell lines for 3 hours. After the culture, IL-1β mRNA expression in HGF was evaluated by reverse transcription-polymerase chain reaction. We found that IL-1β mRNA expression was clearly increased when HGF physically interacted with human lymphoid cell lines, K562 and Ramos, which constitutively demonstrated significant binding ability to HGF but not IL-1β mRNA expression. Moreover, exogenous addition of culture supernatants of the lymphoid cells, which had been cultured in the presence or absence of HGF for 3 hours, did not induce IL 1β mRNA expression in HGF.<BR>These findings indicated that the adhesive interactions between lymphoid cells and HGF should cause signal transduction into HGF and induce the increased expression of IL-1β mRNA in HGF. The present study suggests that HGF would modulate the inflammatory responses in periodontal lesions by interacting with the locally infiltrated lymphocytes.
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