Regulation of histamine production of bone marrow cells stimulated by inflammatory exudate.

概要

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The mechanism for histamine release of bone marrow cells stimulated by the inflammatory exudate, the pouch fluid in air pouch type allergic inflammation in rats, was examined. When bone marrow cells were incubated in the medium containing dialyzed pouch fluid supernatant, histidine decarboxylase activity in the cells and histamine level in the medium were significantly increased when compared with those in bone marrow cells incubated in the medium alone. Addition of an RNA synthesis inhibitor, actinomycin D, or a protein synthesis inhibitor, cycloheximide, into the medium strongly reduced the dialyzed pouch fluid supernatant-induced increase in histamine level in the medium. Inhibitors of DNA synthesis, mitomycin C and cytosine arabinoside, reduced it partially. In addition, it was inhibited by retinoic acid, a differentiation-inducer, but was augmented by sodium n-butyrate, which induces differentiation of bone marrow cells to basophils. Without dialyzed pouch fluid supernatant, retinoic acid and sodium n-butyrate did not affect histamine production. These results suggest that the inflammatory exudate in allergic inflammation has an activity to stimulate histamine production by bone marrow cells by inducing de novo synthesis of histidine decarboxylase which is regulated by growth and differentiation of histamineproducing cells.
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