ISOLATION OF MYCOPLASMA FROM MAMMALIAN CELL LINES AND ITS ELIMINATION BY TREATMENT WITH ANTIBIOTICS

概要

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A total of 13 strains of Mycoplasma were isolated from 15 strains of established cell lines. All of them, except an unidentified strains were of human origin, including 5 strains of M. orale and 7 strains of M. hominis.Each of three cell strains, BHK21, RK13 contaminated with M. orale, and HmLu contaminated with M. hominis, was treated with a culture medium containing gentamicin (200γ/ml), kanamycin (250γ/ml), and tylosin (250γ/ml) or containing kanamycin and tylosin (250 or 500γ/ml each) for 9 days by changing the medium every other day.Mycoplasma was eliminated from RK13 cells even with the medium containing 250γ/ml each of kanamycin and tylosin and from HmLu cells only with the medium containing the three antibiotics. None of these cell lines yielded Mycoplasma during the subsequent 21 passages for a period of 150 days in the absence of the antibiotics used to the treatment. Mycolasma could not be eliminated from BHK21 cells, but the combination of the three antibiotics was more effective for treatment of the cells than that of two antibiotics.The results obtained suggest that the combined use of multiple antibiotics may be promising in eliminating Mycoplasma from cell culture, without giving any damage to the cells.
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