DETECTION OF MEASLES VIRUS IN EGG CULTURE AND STUDIES ON THE PROPHYLACTIC INOCULATION WITH LIVING MEASLES VIRUS

概要

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The method of back inoculation in monkeys or man has been used heretofore to determine whether there has been propagation of measles virus in egg cultures. The virulence of this virus however readily become reduced by passage through eggs so that it is almosst impossible to detect whether propagation has taken place in each individual egg. The authors therefore conducted studies on the method for detecting propagation of the measles virus in the egg culture. Propagation of measles virus was accurately determined by the interference phenomenon with a hemagglutinating virus (the mumps virus was found to be most suited), neutralization of the interference phenomenon with immune measles serum and the complement fixation reaction, with the culture material as the antigen.As stated previously, the egg cultured measles virus becomes almost avirulent and intranasal inoculation of this active virus imparts immunity to children who have not been infected previously with measles. It appears as if immunization of measles has been successfully attained but the number of cases is small so a conclusive statement must be with held until the reproducibility can be further verified.This paper will be published in English in the Med. J. Osaka Univ. Vol. 6, No. 3-4.
日本ウイルス学会の論文