NEWBORN VIRUS PNEUMONITIS (TYPE SENDAI) 2ND REPORT:THE ISOLATION OF A NEW VIRUS, POSSESSING HEMAGGLUTININ ACTIVITY

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In the spring of 1952, an outbreak of newborn Pneumonitis occurred in this University Hospital.In our laboratory, the isolation of virus from 5 autopsic materials was carried out by nasal instillation of lung suspensions to mice. All of the mice died between 3 to 7 days and presented a consolidation of lungs, resembling that of influenza. The pathological findings of lungs were similar to that of newbornes and no inclusion bodies have been smeared. Except pernasal instillation also intracerebral and intraperitoneal injection to mice caused the death of mice and consolidation of lungs.After adaptation to mice, they could be cultivated in amniotic and allantoic cavity of fertile eggs, and after subsequent passage, hemagglutinin (HA) was proved in both cavities. HA was adsorbed to and eluted from chicken erythrocytes. Heat stability of HA was so feeble when compared to another virus, belonging to MNI. Receptor gradient of the virus was examined and it was arranged between NDV and FMI.HA-inhibition test was performed by several specimens of monovalent rooster serum, i.e. of this virus (Fushimi), Mumps (Enders), Newcastle (Fukumi), Influenza A (PR8, WS), A' (FMI, Lépine and Matsumoto), B (Lee, Seattle and T14) and C (1233). No other serum could inhibit this HA, except the correspondent serum of the agent. Beside this Akitsugu and Tochigi strains, both isolated from adult's influenza in other laboratories were proved to possess the same antigenic structure with this virus. The neutralization antibody was proved with convalescent serum of patient and immun serum of rabbit.Recently we could find again a new sporadic case, and isolated the same virus from throat and the elevation of antibody against this virus in paired serum of this patient was proved by HA-inhibition. test. Further research proved the enzootic nature of this virus i.e. the same virus was isolated from normal laboratory mice. But all of mice examined have no antibody against this virus.Intranasal instillation of the virus to a volunteer caused a fever of the saddleback type and high elevation of antibody. The virus was also recovered from throat 2 times after onset.The purification studies of the virus by the supercentrifuge revealed a spherical particle 150 to 200mμ in size resembling rather to mumps virus and NDV than influenza virus.
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NEWBORN VIRUS PNEUMONITIS (TYPE SENDAI) 2ND REPORT:THE ISOLATION OF A NEW VIRUS, POSSESSING HEMAGGLUTININ ACTIVITY

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