Lymphocyte subsets in the peripheral blood of donors and into the collected bags in the platelet and the granulocyte apheresis using the IBM 2997 cell separator.

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In the platelet apheresis and granurocyte apheresis used by the cell-separater: IBM 2997, the changes of absolute number of lymphocytes and lymphocyte subsets were investigated in comparison with pre- and post-apheresis in the peripheral blood of donors. Also, lymphocyte subsets into the bags collected from donors were examined.In the platelet apheresis, absolute number of lymphocytes and precentages of lymphocyte subsets were not changed in the peripheral blood of donors.However, in the granulocyte apheresis, after injection of hydrocortizone (200mg), percentages of lymphocyte, OKT3 and OKT4 positive cells in the peripheral blood of donors were decreased significantly compared to pre-apheresis.On the other hand, OKT4/8 ratio into bags collected by granulocyte was more decreased significantly compared to platelet apheresis bags.From these results, we strongly feel that the prevention of lymphocyte contamination and calculation of OKT4/8 ratio into the collected bags will be important to avoid the allergic reaction and GVHD.
一般社団法人日本輸血・細胞治療学会の論文