Studies on Interaction between Genetically Engineered Microorganisms and Their Parental Strains
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概要
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In this study, effect of environmental factors on interaction between genetically engineered microorganisms (GEMs) and their parental strains was investigated. Some species of<I>Escherichia coli</I>strains were supplied to analysis of competition of GEMs and their parental strains, but finally, <I>E.coli</I> HB101/pBR325 and<I>E.coli</I>HB101 were selected to investigate their interaction. In batch culture, the specific rgrowthate (μ) and Ks were measured respectively, and in the serial transfer culture, the rate of maximum individual number (N<SUB>max</SUB>) and their continuous change were observed. As a result of this study, it was suggested that the rate of individual number of GEMs decrease in long term culture with their parental strain. So, it was considered that the prosperity and decay of GEMs and their parental strains are almost same or GEMs are not able to survive longer than their parental strains in any environment.
- 日本水処理生物学会の論文
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