IMMUNOCYTOCHEMICAL STUDY OF AMYLOID β PROTEIN PRECURSOR IN MOUSE AND RAT BRAINS:LOCALIZATION IN THE NORMAL BRAIN AND CHANGE WITH AGING AND BRAIN DAMAGE

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We studied the immunocytochemical distribution of amyloid β protein precursor (APP) in the nervous systems of the mouse and the rat, and changes with aging and brain damage induced by intraventricular injection of kainic acid in the rat brains. We used three different antisera against synthetic peptides of APP and one monoclonal antibody against APP. On Western blot analysis, both antisera against carboxyl-and amino-terminal regions of APP recognized the same 106-122 KDa proteins from mouse brain. Immunocytochemically, APP immunoreactivity was located in all types of neurons and some glial cells in both the central and peripheral nervous systems. In the brains of normal aged rats, APP accumulated in swollen neurites, most of which were axons. These swollen neurites appeared throughout the central nervous system. In the rat brains injected with kainic acid, APP positive dystrophic neurites appeared around the track of the cannula after 3 h, neurons near the lesion showed increased APP immunoreactivity after 6 h, and reactive astrocytes expressed APP with Kunitz-type protease inhibitor domain in the lesion and ipsilateral hippocampus after 3 days. APP was expressed by neurons and glial cells throughout nervous systems, and these distributions changed with aging and brain damage.
北関東医学会の論文