COMPARATIVE STUDY OF ESTROGEN RECEPTOR ASSAY IN HUMAN BREAST CANCER USING 16α-<SUP>125</SUP>I-ESTRADIOL AND <SUP>3</SUP>H-ESTRADIOL
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Estrogen receptors (ER) were determined in 50 human mammary tumor tissues by the dextran-coated charcoal method using 16α-<SUP>125</SUP>I-estradiol-17β (<SUP>125</SUP>I-E<SUB>2</SUB> Kit) and the sucrose density gradient method using <SUP>3</SUP>H-estradiol-17β (<SUP>3</SUP>H-E<SUB>2</SUB>). In this study, if a tumor tissue contained more than 5 fmol of ER per mg cytosol protein, it was judged to be ER-positive and if 0 to 5 fmol was present, as ER-negative. The coincident rate of positivity and negativity of ER between the two methods was 90 % (45/50) and the correlation coefficient for the binding sites of ER was r=0.863 in 19 ER-positive cases.<BR>These results showed that in ER assay, the <SUP>125</SUP>I-E<SUB>2</SUB> Kit was as useful as <SUP>3</SUP>H-E<SUB>2</SUB>, and that the former was rapid, simple and practical.
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