STUDIES ON SECRETOR SPECIFIC H (Se) ANTIGEN OF WATER SOLUBLE BLOOD GROUP SUBSTANCES:1. ANTI-H (Se) PRECIPITIN IN EEL SERUM
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The presence of two precipitins against blood group H substances from human gastric linings and ovarian cyst fluids was demonstrated in natural eel serum by immunodiffusion techniques in agal gel. One of them which form the precipitation band to antigen side was the known anti-H precipitin containing anti-H agglutinin activity. Reaction of the anti-H precipitin with H substance was inhibited by L-fucose. This anti-H precipitin reacted well with H substance from hog gastric linings but did not show clear precipitation bands with H substances from saliva, meconium, semen and intestines. The other precipitin which form the precipitation band to antiserum side in agar gel reacted well with H substances from human secretions and tissues but not reacted with hog H substances from gastric linings and submaxillary gland. This precipitin did not agglutinate O red cells and was not absorbed by O red cells. The reaction of the precipitin with H substances was not inhibited by L-fucose. Because this precipitin reacted with water soluble H substances from human "secretor", the antigen named H (Se) and antibody anti-H (Se).
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