EFFECT OF COMPOUND 48/80 ON HISTAMINE RELEASE IN ANAPHYLAXIS
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Histamine release in anaphylaxis was measured spectrophotofluorometrically by immersing passively sensitized guinea-pig small intestines in Tyrode solution containing crystalline ovalbumin. The amount of histamine release was calculated as the free base and was expressed in μg, per g, of dry tissue weight. And the effect of Compound 48/80 was investigated.<BR>1. Histamine release from sensitized small intestines by the antigen (2.5×10<SUP>-5</SUP>g/ml) was 0.319±0.024μg/g.<BR>2. Small intestines were treated with Compound 48/80 in concentration of 10<SUP>-3</SUP>g/ml for 15 and 30 min, and then sensitized. Histamine releases from them by the antigen increased significantly in compare with those by the antigen alone ; 0.716±0.170μg/g in the former and 0.767±0.231μg/g in the latter.<BR>3. Small intestines were treated with Compound 48/80 in concentration of 10<SUP>-5</SUP>g/ml for 15 min and then sensitized. Other small intestines were treated vice versa. Histamine releases from them by the antigen were 0.230±0.040 and 0.390±0.049μg/g, respectively. The value in the latter showed significant increase when compared with that in the former.<BR>4. Histamine release from sensitized small intestines in anaphylaxis which were preteated with Compound 48/80 of various concentratins for 15 min increased along with the increase in concentration of Compound 48/80.
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