STUDIES ON THE STANDARDIZATION OF CLINICAL HAEMOGLOBINOMETRY
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概要
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The purpose of the present paper is to standardize the clinical hemoglobinometry. The auther adopted three methods of Hb determination, that is iron determination, gas-metric method and photometric method on the same sample. Critical descriptions of each methods were described in detail with its historical review. To obtain correct absolute value of Hb, the recovery of iron from Hb was estimated by our original method. Hb with Fe<SUP>59</SUP> was synthesized in vivo by injecting Fe<SUP>59</SUP> labeled plasma into anemic rabbit prepared by blood letting. The wasted Fe<SUP>59</SUP> during the chemical process of determination was estimated accurately by the method.<BR>The auther found the complete recovery of the Hb iron if corrected by using control sample. Thus the auther could obtain the following results :<BR>(1) The Hb value by iron method showed about 1.7% higher than that by the gas-metric method and this is statistically significant.<BR>(2) The Hb value by the gas-metric method and photo-metric method gave the same value, though there were some discrepancies each other.<BR>(3) The difference of the results by iron estimation and photo-metrical determination using E<SUB>540</SUB>= 0.143 was 2.3%.<BR>(4) To determine total Hb by MHbCN method, E<SUB>540</SUB> of 0.146 was established.<BR>(5) Some correction should be necessary, if the most recently established molecular weight of Hb is applied. For this purpose, international co-operation of the research workers of this field is further needed. At the present time the auther suspended the final decision.
- 北関東医学会の論文
北関東医学会 | 論文
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