Influence of Excessive Ascorbic Acid Dose on Liver Microsomal Mixed Function Oxidase System in Guinea Pigs
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概要
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In the first experiment, guinea pigs were fed diets containing graded levels of ascorbic acid (AsA) from deficient to excess (0, 6, 60, 600, 6, 000, and 12, 000mg/kg of diet) for 15 days after pre-feeding with a low AsA diet (60mg/kg) for one week. Pentobarbital sleeping time measured on day 9 was shortened in the 600- to 12, 000-mg AsA groups as compared with the two AsA deficient (0 and 6mg) and 60-mg AsA groups. Liver microsomal cytochrome P-450 content increased with a dose-response relation to the dietary AsA level except in the excess AsA group (12, 000mg/kg). However, cytochrome b5 content and NADPH-cytochrome c reductase activity showed no significant response. Activities of aminopyrine N-demethylase and aniline hydroxylase showed the same response as seen in cytochrome P-450 content, but to a lesser extent. In the second experiment, guinea pigs were fed the diet containing the normal level of or excess AsA (300 or 24, 000mg/kg of diet, respectively) for 4, 9 or 15 days after a pre-feeding with the normal AsA diet for one week. No significant differences in the time courses of those responses described above were shown between the two AsA groups. In conclusion, the liver microsomal mixed function oxidase activity did not change even transiently during a short period after administration of a large excess of AsA such as 24, 000mg/kg of diet, and 300mg AsA/ kg of diet is enough to ensure normal levels of the liver microsomal mixed function oxidase system in guinea pigs.
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