Use of Phospholipase D and Choline Oxidase for the Enzymatic Determination of Calcium Ion in Serum
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概要
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A novel enzymatic method for the determination of calcium ion (Ca2+) is described. The principle of the assay is to determine the degree of activation by Ca2+ present in a given solution of a Ca2+-dependent enzyme, phospholipase D, which is subsequently assessed in terms of the amount of choline produced. For the latter determination, choline oxidase and peroxidase are used in combination, leading to the color production, which can be simply quantified by measuring the increase in absorbance at 500nm with time. The present method is highly Ca2+-specific, accurate, and reproducible, giving results in reasonably good agreement with those obtained by the Ca2+-selective electrode method. The results of the enzymatic method are not influenced by any change in pH of the serum samples that may occur during the storage of these samples.
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日本酸化ストレス学会 | 論文
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