Nutritional Studies on Chlorella (Report 14):On the Isolation and Extraction of Protein of Chlorella, Chlorella V ulgaris (Part 1) Extraction with Some Reagents and Releasing with Autolysis of Algae

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Extractions of the protein from fresh frozen algae with some reagents and change of the releasing of protein with autolysis were studied. On the protein extraction, algae was ground sufficiently with a little amount of reagent and sea sand in mortar and soaked in the reagent. The supernatant obtained from this treatment after centrifugation at 4000r.p.m. for 20 minutes was used to determine, the nitrogen extracted. These results are shown in Table 1. Also, the results of changes of releasing of protein with autolysis of algae are shown in Figure 1. Considering from the results shown in the Table 1, NaOH seems most extractable of all the reagents employed, the extracted put being 50% of total nitrogen of algae. On the other hand, only a little protein-nitrogen was extracted. With autolysis for 50 hours, 7096 of total nitrogen of algae and 50% of total protein nitrogen became soluble. Figure 2 also shows the results of change of protein releasing with autolysis by paper electrophertic diagrams. Figure 3 presents the electron-microscopic photography of observation of these process of autolysis.
特定非営利活動法人日本栄養改善学会の論文